Fatigue is a widespread issue that impacts individuals across various domains of life, from learning and work to sports and driving. Fatigue is also a prevalent symptom of many chronic diseases. In this presentation, we will share the latest insights from our multimodal neuroimaging studies, which explore the distinct neural correlates of subjective fatigue experiences and objective cognitive fatigability in the human brain. Understanding both the subjective and objective aspects of fatigue is crucial for enhancing health and safety, devising novel treatments, optimizing performance, and improving the quality of life for both healthy individuals and clinical patients.